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In contrast, around 30% of sites enriched in the mutant show only very low occupancy at other stages.

Further, the C48A/C139A double mutant shows only monomeric behaviour.

A significant increase in Cox-2 expression was observed with R248Q (DNA contact mutant) and V272M (temperature-sensitive mutant), whereas R175H (conformation mutant) showed only minimal effects.

In keeping with this notion, we found that the pri2(C4343A) mutant allele exhibited synthetic growth defect with sod1Δ; the double mutant is lethal at 30°C while each single mutant showed only mild growth defect (Fig. 7C).

Since the LinBUT I134V/A247H (=mutantmutant showed only weak LinBMI-type activity in our previous study (Ito et al. [2007]), cumulative mutations at the positions A112, I138, and M253 were introduced into the M2-1 mutant.

Interestingly, fadD1 mutant showed only increased swarming motility.

Mortality assays performed with the bscN mutant showed only a partial difference with K56-2 wild-type (Figure 5C).

In the latter species, even a ppsApckA double mutant showed only a marginal degree of attenuation indicating that gluconeogenesis plays, at most, a minor role for Salmonella virulence.

We found that the apc1 mutant showed only a 1.7-fold difference in centrosome and spindle misorientation (Figure 4C), indicating that the apc1 mutant is partially defective in the centrosome orientation checkpoint.

The pse1-1 kap123Δ mutant showed only a slight increase in cytoplasmic staining of GFP-eS26.

Consistent with the GFP-TRAP experiment, the K7A/D69A mutant showed only slightly reduced binding to the GFP-ubiquitin-coated beads.