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The roots of the wav3 mutant show a short-pitch pattern on inclined agar media and a positive gravitropic response.
However, leaves infiltrated with the XacPNP deletion mutant show a more pronounced decrease in photosynthetic proteins while no reduction in defense related proteins as compared to the wild-type pathogen.
Figure 4A shows that although egr3 mutant show a slight increased susceptibility to this challenge compared to wild-type flies, however this was not as pronounced as spz and PGRP-SA mutants.
Since previous reports have described reduced ligand-induced CXCR4 internalization in cells from patients with WHIM syndrome [28], we tested whether HeLa cells expressing the WHIM-associated CXCR4 mutant show a similar impairment.
Previous results [8] detect no more than two parameters from the hmg1 and hmg2 mutants with normal distributions on one side at P<0.0001, suggesting that our high-content image-profiling method can detect mutants of genes with major cellular roles even if the mutant show a weak phenotype.
The comparison of the H N HSQC NMR spectra obtained for wild-type and each mutant show a similar dispersion of signals, indicating that the overall fold of the native protein is maintained.
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The best mutant showed a 4.3 °C increase in its T5015.
An S174A mutant showed a similar degree of autophagy impairment as an Atg31 deletion mutant.
The Glu56Ser (E56S) mutant showed a 1.6-fold higher activity than the wild-type enzyme.
H209N was the most thermostable mutant, showing a Tm of 71.3 °C.
However, the mutant shows a different orientation in the hinge region that connects two domains of the protein.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com