If these export proteins participate in FFA export, the associated knockout mutants should show reduced extracellular FFA concentrations and possibly an increased toxic response to FFA production.
For a recessive mutation, any linked SSR marker should show only the band corresponding to the mutant parent allele in the mutant pool sample, while the wild-type pool should carry both the wild-type and mutant parental alleles (Fig. 4), although there is often a faint wild-type band in the mutant pool (Appendix 1, Note 10).
A linked marker should show an overrepresentation of the mutant parental allele in the mutant samples.
Similarly, they should show that the S473A mutant no longer shows the phosphorylation shift on the gel.
2) The quantitative data for NPC cap formation in a bud6∆ mutant should be shown in Figure 2. 3) In Figure 11C, another interpretation of the data is that GCN5 and SPT20 are required for the RLS extension caused by deleting FOB1, possibly because the gcn5∆ mutant already has reduced ERC accumulation (as shown in Figure 3C).
2) The quantitative data for NPC cap formation in a bud6∆ mutant should be shown in Figure 2. As requested by the reviewers, we added these results to Figure 2. Similar to wt cells, the NPC signal is twice as strong around the plasmids compared to the residual nuclear area.
Deletion of genes involved in assembly should show allele specific defects with SPC110 mutant spc110-220, wasch was previously determined to be defective in assembly [8].
Additional comments are below: Figure 1 should show quantification of multiple blots for PER2 cycling in the LaminB1 mutant and overexpression backgrounds.
If the ORF of the tri3 was replaced successfully by the hph cassette in mutants, transformants 2, 5, 6, and 10 (lanes 13, 16, 17, and 21, respectively) should show the expected 3.6 kb band (reduced by approximately 0.7 kb).
