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Differences in glutathione contents between the wildtype and the pad2-1 mutant should give detailed insight into the roles of temporary and permanent glutathione depletion on pollen germination in vitro.
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Our goal was to generate 10,000 fission yeast mutants with unique and non-redundant barcodes, which should give a 76% chance to cover all annotated genes (details in Materials and Methods).
7b, 32b Furthermore, simulations should give as much atomistic insight as possible into mutant protein defects.
We have screened 8 million cells with retroviral transduction efficiency of 20%, which should give rise to 1.6 million independent clones of cells containing mutant Bcr-Abl.
We reasoned that combining the two mutations E144R and R225E both of which conferred >2-fold increases in the UUG AUG ratio, should give an additive effect and increase the UUG AUG ratio more than either of the single mutants.
At the very least, the authors should give more information relating to the hypothesis that suppression of pectin degradation in the triploid PEG mutants contributes to increased viability.
That should give everyone pause.
They should give us hope.
It should give people hope".
This should give us pause.
We should give it up.
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