Sentence examples for mutant sequences was from inspiring English sources

Structural distance between wild-type and mutant sequences was calculated from base pairing probability matrices (Sabarinathan et al. 2013).

The structural distance (d max ) between wild-type and mutant sequences was calculated from base pairing probability matrices [ 33].

A multinomial distribution analysis was used to determine if the presence of these mutant sequences was a statistically significant event, resulting in a p value of 4.2 × 10-9.

F3 and F5 mutant sequences were used for Flpe-mediated cassette exchange, and m2 and lox2272 mutant sequences were used for Cre-mediated cassette exchange due to their high incompatibility with wild-type sequences.

The mutant sequences are numbered from 1 to 12. HAuCl4, sodium tris-citrate, NaOH and HCl were purchased from Xilong Chemical (Guangdong, China).

All mutant sequences were verified by DNA sequencing.

However, even under selective pressure from monoclonal antibodies, very few escape mutant sequences were observed for M2e [30] suggesting that this region is biologically constrained.

Mutant sequences were obtained by site-directed mutagenesis using PCR from 30 ng of pGL3 vector (Promega), pfu DNA polymerase (Stratagene) and the following primers (Eurogentec): 333-5'-GGAAGATGGAACCGCTGGATAGCAACTGCATAAGG-3'-CCTTATGCAGTTGCTATCCAGCGGTTCCATCTTCC-3CTTCC-3'.

Mutant sequences were confirmed by PCR assay.

Regenerated mutant sequences were used further for mutant modeling.

All mutant sequences were confirmed by DNA sequencing.