Sentence examples for mutant sequences based from inspiring English sources

All luciferase reporter constructs containing both wild type and mutant sequences based on the VEGF promoter were supplied by Dr. Amit Maity (University of Pennsylvania). 1 µg of each construct was transfected into freshly-isolated human monocytes using the Amaxa Nucleofector Transfection System as per the manufacturer's protocol (Amaxa).

Bini et al. [ 16] generated a library of 994 mutant DNA sequences based on the sequence of a well-known and characterized 15-mer thrombin-binding aptamer (TBA) for the in silico selection.

Several splice site prediction tools compare the predicted mutant sequence to a consensus sequence, based on a set of functional acceptor or donor splice sites.

3 lines were chosen for whole genome sequencing based on phenotype: J318 (mutant frequency 8%%), J328 (mutant frequency 4.3%%), and J327 (mutant frequency 2%%).

We decided to prioritize candidates for sequencing based on gene structure and known mutant phenotypes; ENU mutants are biased towards mutations in genes with longer coding sequences and more exons [12].

Sequencing of the insertion sites indicates that three insertion sites in the two LS mutants contain repetitive sequences while insertion sequence in the control mutant, which does not have the dramatic flower phenotype, is not a repetitive sequence based on GenBank search.

The proposed approach has been successfully implemented for the identification of the single-base mutant sequences in the human KRAS gene with a detection limit of 1.8 pM.

Structural distance between wild-type and mutant sequences was calculated from base pairing probability matrices (Sabarinathan et al. 2013).

The structural distance (d max ) between wild-type and mutant sequences was calculated from base pairing probability matrices [ 33].

Wild-type and mutant sequences were subsequently cloned into pCS2+ based expression vectors that were used for mRNA synthesis and transfection.

Therefore, if next-generation sequencing is applied to analysis of a heterogeneous population of various mutant sequences, it is necessary to discriminate between true bases as point mutations and errors of base call in the observed sequences, and to subject the sequences to error-correction processes.