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A mutant sequence where GGAA was changed to AAAA was also used.
To exemplify SADI service composition, we present an example of a query which in natural language reads: "Retrieve all mutated proteins, together with their 3D-structure information and mutant sequence, where mutations had a positive impact on haloalkane dehalogenase activity".
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The mutant sequence of cloned PCR products was confirmed by DNA sequencing.
(b) Typical sequencing traces are shown for exons 9 (right) and 20 (left) with cancer mutant sequence (bottom) and normal wild-type sequence (top).
The 173-bp ssODN donor template is presented under the genomic DNA with a R229Q mutant sequence in green.
Sequencing of cDNA from cells infected with this last mutant demonstrated that the parental mutant sequence was retained and that genotypic revertants to the wild-type as well as new mutant sequences were generated.
Sequence analysis revealed only the mutant sequence.
Kd for mutant sequence was 649 nM.
The mutant sequences were used in the mutant 3′-UTR constructs.
Wild-type and mutant sequences were compared to identify putative resistance mechanisms.
All mutant sequences were verified by DNA sequencing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com