For ST mutant, apical segments were exclusively used to obtain normal shoots without tuberization.
To enhance functional surface expression of GluClβ receptors, point mutants or chimeras with GluClα segments were made in regions of the receptor previously shown to affect Cys-loop receptor trafficking and expression levels (SI Figure S2).
However, both the shape and the length of the tracheal dorsal trunk segments were abnormal in this mutant (Fig. 2A–D').
Consistent with photoreceptor alteration, PNA labelling of cone outer segments was reduced in mutants, which also showed absence of rhodopsin at P120 (Fig. 3A).
Viruses carrying the WT or AUG mutants of the M segment were rescued in BHK/T7-9 cells, and viral stocks were produced on Vero E6 cells.
Consecutive residues in putative transmembrane segments are mutated to cysteine, one at a time, and the mutant proteins are expressed in heterologous cells.
This almost complete hydrolysis of cellulose in inflorescences of c4h-2 and ccr1 mutants during saccharification was also visually noticeable; the structure of the stem segments was completely lost.
However, only the two anteriormost segments are formed from Nv eve stripe 6 (6andnd 6b) in Nv hb mutant embryos.
Today, lighter segments are employed.
