We have conducted functional microarray and expression pattern screens based on the Foxa2 mutant embryo.
In addition, para bss1 has not responded to seizure suppressor mutations identified in screens based on the Drosophila mutants eas and sda.
The insertion mutants were screened based on the protocol reported by Heap [24], Intron insertions were verified by PCR using primers Cac1494B and Pex1494E flanking the target site and then PCR products were purified and sequenced, the sequence has been deposited in (Genbank number: HQ257448).
The mutants were screened based on the distribution of Arr2-GFP by confocal live imaging under water-immersion lens using 3xP3-RFP as the wild-type marker, as previously described for the screening of insertional mutants (Satoh et al., 2013).
Transposon insertions in fakA were first isolated in a large S. aureus transposon (bursa aurealis-bearing) insertion mutants library screen based on an increased resistance to an antimicrobial peptide [ 27].
Using either active Mutator (Mu) transposable elements or EMS mutagenesis, genetic screens based on these paramutation systems have identified several mutants.
Forward genetic screens based on seed composition, above-ground, or below-ground visual phenotypes identified 216 mutant plants that were selected for genomic analyses.
From this screen we retrieved 20 unique gfp mutants, which were combined for further analysis with eight additional unique, uncharacterized gfp mutants recovered in a separate screen based on the same GFP reporter gene (Sasaki et al. 2012) (Table 1).
From a screen of approximately 280,000 M2 seedlings (approximately seven M2 progeny were tested from each M1 plant) (Haughn and Somerville 1990; Jander et al. 2003), we retrieved 20 unique gfp mutants, which were combined for further analysis with eight additional unique, uncharacterized gfp mutants identified in a separate screen based on the same GFP reporter gene (Sasaki et al. 2012).
Unlike the previous screen based on isolating mutants of DsbG that alleviated a general copper-induced oxidative stress, in this article we present a new screen based on the folding of a mutant PhoA.
