Sentence examples for mutant screening of from inspiring English sources

Jørgensen and colleagues performed a mutant screening of barley and isolated over 20 loss-of-function alleles of the Mla12 gene, which confers resistance to powdery mildew, and only three additional mutants in two other loci (RAR1 and RAR2) [ 43, 44].

From a mutant screen of plants containing a GFP tagged component of the Casparian strip we identified a transcription factor, MYB36 that is required to activate expression of genes involved in Casparian strip formation.

Here we describe a symbiotic mutant screen of fast neutron and EMS mutagenized Medicago truncatula plants carried out to identify additional symbiotic mutants.

This characteristic could be useful as a simple assay, e.g., for mutant screening, to study regulation of this pathway in more detail.

KRYPTONITE was identified in a mutant screen for suppressors of gene silencing at the SUPERMAN locus (Jackson et al., 2002).

Ecs was originally identified in Bacillus subtilis in a mutant screen for decreased secretion of overexpressed α-amylase [6], [7].

Still, a second mutant screen for suppressor mutants of GPXH overexpression in strain 21B4 was performed using an additional plasmid containing the GPXH promoter in front of the nitrate reductase gene (data not shown).

The addition of sorbose (0.4 %) restricts the growth of mycelia facilitating more number of mutant screening per plate (Chand et al. 2005).

For initial mutant screening, 16 seeds each of 36 M2 line were put in 96-well PCR plates that were floated on a 0.5 mM CaCl2 solution in a 10 l container at 25 °C.

Through genome-wide mutant screening and analysis, dozens of novel genes were discovered to be involved in the process.

Figure  1A showed the results of mutant screening by gradient PCR using D112K forward and reverse primers which contained two designed mutations.