Exact(2)
It is suggested that the authors dilute the double mutant samples such that the 4h lactate signal of the two strains are equal and then analyzed the ubiquitination level of Jen1.
It is suggested that the authors dilute the double mutant samples such that the 4h lactate signal of the two strains are equal and then analyzed the ubiquitination level of Jen1. 1) The authors speculate that Jen1 gets actively de-ubiquitylated after internalization and therefore requires another round of ubiquitylation in the TGN in order to be directed to the vacuole.
Similar(58)
The methods must be sensitive enough to detect such low-frequency mutant samples.
A bacterial mutant with enhanced PHB production can be distinguished from randomly mixed samples such as random mutagenesis by error-prone PCR in a high-throughput manner using LipidGreen1.
Rhodopsin mutant samples and buffer F were mixed with 1 mM 4-PDS such that the final solution contained 0.5 µM rhodopsin and 25 µM 4-PDS.
As we found such a high number of Hif-1α binding sites in vhl mutant samples (5177), we were concerned that this could lead to a high number of false associations with genes in their vicinity.
Comparative studies can be used with two or more samples, such as active vs inactive, diseased vs healthy, treated vs untreated, wild type vs mutant, and so on.
TP53 mutant samples largely overlap the basal-like samples and the ER-negative samples.
Each change was re-evaluated against wildtype and mutant samples.
A linked marker should show an overrepresentation of the mutant parental allele in the mutant samples.
Mutant samples were confirmed by PCR analysis of sall expression.
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