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Although affected by the same genetic defect the WRN mutant samples revealed a high variability, which might be explained by the nature of the genetic defect.
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qPCR analysis of the relative copy number of the attP site in the rsmA mutant versus the WT samples revealed that there was a 17-fold increase in the frequency of excision in the rsmA mutant; although there was no increase in copy number for or4140 (a cargo gene), suggesting that the prophage does not replicate within the cell.
Dozens of blood samples revealed nothing untoward.
The samples revealed high levels of lead.
But Ormandy says his samples revealed highly sensitive data.
Four samples revealed a strong cytoplasmatic staining.
Only two samples revealed sheathed microfilariae.
A few samples reveal the differences.
Environmental sampling revealed widespread contamination.
However, we detected no obvious alteration in the fraction of Drp1 that distributes with mitochondria in park25 or PINK1B9 mutants relative to wt controls (Figure 2E), although this experiment revealed that the higher MW form of Drp1 appeared to preferentially localize to mitochondria in both wt and mutant samples.
Volcano plots of p values against gene expression fold changes revealed that the embryonic epsilon globin gene (Hbb-y) was by far the most affected gene, with an upregulation fold of more than 200 in all mutant samples (Fig. 5A).
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