If the mutants showed the same wetting as the wild types, the probability of an individual mutant sample showing Wenzel wetting would be 0.645 and the probability of 14 mutant samples (out of 14) showing Wenzel wetting can therefore be calculated as 0.002 (exact value from binomial test).
The length and NAT type in each mutant sample of NAT-siRNAs were further analysed (Fig. 2C).
The ratios of mutant samples to the wild-type control were normalized by a trimmed geometric mean [ 11, 32, 33].
We recorded 2D C C correlation spectra of the mutant samples to verify that the protein refolded and was properly reconstituted into lipid bilayers.
Interestingly, the non-mutant sample of the father (AG15693) revealed an increase of variability compared with the two daughters (AG15694, AG15695), likely reflecting the fact that, besides presenting the HGP phenotype, the father was 28 y older at sample acquisition.
The average (AVG) expression ratio of the mutant sample to the wild-type control was calculated from 12 replicates together with the respective standard error (SE), a standard t-test result (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; *****, P < 0.00001 probability in Student's t-test).
We also performed simulations, using random mutant sampling, to determine the minimum number of mutants per samples that would be sufficient to achieve a consistent mutation spectrum in the BaP group.
The mutant samples each consisted of DNA from one FN individual, with no bulking or pooling of mutant samples.
The relative level (or fold change) of a protein in the knockin mouse represents the ratio of the average value of the two mutant samples ([114+115]/2) to that of the two wild type samples ([116+117]/2).
