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Microarray analysis of a Δstk1 mutant revealed increased transcription of additional exotoxins.
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Compared to wt, the three mutants revealed increased ascorbate peroxidase activities in high and low CO2.
Gene expression analysis of these mutants revealed increased levels of Col1 and Loxl2 and a decrease in Snail1, a marker for migrating neural crest (Fig. 6I).
However, examination of IFM ultrastructure from aged HtrA2 Δ1 mutants revealed increased numbers of defective mitochondria, displaying reduced electron density and open cristae, compared with wild type and HtrA2 Δ1 rescued controls.
Histological analysis of mutant placenta revealed increased mineralization and mutant embryos were found to be approximately 25% smaller than wild-type littermates.
Real-time PCR (RT-PCR) and immunofluorescence analyses in mutant HF-SCs revealed increased transcription of the Ink4b/ Ink4ArfArf gene locus, which encodes cell cycle inhibitors p16, p15 and p19 [ 76].
In accordance with the sleep phenotype, wheel-running and drinking analysis revealed increased activity of Prkg1 mutants during the day, when rodents are usually not active.
Interestingly, the Western blot results revealed increased protein levels for some mutant forms that did not show an increase in activity (e.g., C94S).
Quantification of cell-cell connections from a longitudinal view of collecting ducts (supplementary material Fig. S4A) revealed increased 4-lateral4-lateralthens of the mutants (supplementary material Fig. S4B).
Our phenotype microarray analysis revealed increased sensitivity of the S. aureus Newman Δ vraSR mutant to a wide range of β-lactam antibiotics.
Microarray analysis of selected laboratory mutants with fabI promoter region mutations, grown in the absence of triclosan, revealed increased fabI expression in three out of four tested strains.
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