Other mutants retain normal germ plasm, but the PGCs fail to form (Jongens et al., 1992; Robertson et al., 1999).
However, because both AaegTRPA1 -/- and AaegGr19 -/- mutants retain normal attraction to warmth, this aspect of heat-seeking must rely on other thermoreceptors, still to be identified.
This latter observation is entirely consistent with previous work showing that raptor S859A and S863A mutants retain normal capacity for interaction with 4E-BP1, buthatat mTOR activity associated with the S863A mutant is severely blunted [ 43].
Under this oxidative stress, wild Nm23-H1 partialostlost enzyme activity, and the H118F mutant, the active site mutant, had no enzymatic activity, but C109A mutant retained its normal activity, as did the positive mutant.
Interestingly, one type 2B mutant, R167Q, was unstable yet retained normal HIF-2α regulation in our assays, signifying that deficiencies in stability and VHL function are not synonymous.
Moreover, a number of mutant HbpR proteins with slightly different scans still retained normal induction potential.
Six cancer cell lines retained normal copy numbers.
After extraction of phospholipids and conversion into the corresponding fatty acid methyl esters (FAMEs), we observed the formation of cyclopropanated FAMEs suggesting that the mutant retained some of the normal activity in vivo.
All the mutants retained the normal ATPase activity of gp96 (Fig. S2), suggesting that the mutations did not affect their native conformation.
The BFL1ΔC K101EK102E mutant lost virtually all capacity to permeabilize apoptotic-like LUV whereas the K146EK147E mutant retained most of this ability (Fig. 4f), while both mutants efficiently inhibited BAX permeabilizing activity in healthy-like LUV (Fig. S4d).
