For mutants represented by single alleles, we used high-throughput SNP markers and genetic recombination to map the mutation to a 2- to 3-Mb region (~10% of a chromosome arm), which is usually sufficient to restrict the number of candidate causative coding mutations to one or two.
A myc tag was added to each of these SOCS3 mutants, and exogenous SOCS3 mutants were represented by the detection of myc tag.
Class 3 mutants are represented by three loci, each of which shows reduced VPC induction.
In RCC10 cells, type 1 VHL mutants were represented by constructs coding for pVHL with a deletion of amino acid residues 114 to 178 (del 114 178), a double point mutant RC161/2QW, the L188Q mutation, as well as control cells infected with a retrovirus lacking any VHL sequence.
The osu1-2 allele was also used for DNA amplification in order to verify the mutant gene was represented by At1g78240.
To attempt to separate these functions of Ctf4, we sequenced and characterized a set of nine CTF4 mutant alleles that are represented by four missense mutants and five nonsense mutants, isolated in the original CTF screen (Spencer et al. 1990).
The double mutant represents thermodynamically most stable version among them.
The essential genes identified here are represented by mutant strains, many of which have more than one mutant allele.
Group B is represented by mutant strains PHB-4, H16 ΔptsMHI, H16 ΔptsM, H16 ΔptsH, H16 ΔptsI, H16 ΔptsH ΔH16_A2203, H16 ΔptsMHI ΔfrcACB, H16 ΔptsMHI ΔnagFEC, HF39 ptsmob:Tn5::mob, and HF39 ptsI::Tn5::mob (Figure 1b).
In immunoblots, the mutant huntingtin protein was represented by bands of noticeably lower intensity compared with those of mouse huntingtin.
