Compared with cysteine mutant G121C/Basal mutant, the replacement of six natural cysteine residues by alanine led to an increase of A280/A500 ratios from 1.8 to 6.4 (Figure 3).
In addition, we generated a conditional PMR1 mutant by replacement of the promoter region of PMR1 with a tetO promoter that is active in the absence of doxycycline and prevents gene expression upon addition of 10 μg/ml of the chemical.
This data contrasts the data obtained from full-length Foxp3 mutants where replacement of RKKR residues were found not to alter the kinetics of nuclear transport (Fig. 1, mutants A C).
In both double mutants, the replacement of Ile10 led to narrowed acceptance of alternative substrates.
Kinetic analysis of the different mutants revealed replacement of D779 with Tyr (D779Y) or Trp (D779W) significantly decreased the overall rate of the PRODH P5CDH channeling reaction.
To relax both the assumption of a time-invariant selective advantage and the assumption that successful establishment of a mutant leads to fixation of the mutant lineage (including replacement of the resident lineage), we now consider a more complex epidemiological model that explicitly incorporates the dynamics of susceptible hosts ('Materials and methods').
TRAIL mutant with replacements of Cys230 with Ala (TRAILC230A) negligibly formed trimeric structure and showed no apoptotic activity.
Selective silencing of mutant SOD1 [ 28] or replacement of mutant by wild-type microglia [ 29] in the mutant SOD1 mouse model strongly point to a central pathogenic role of microglia.
Hanks, M., Wurst, W., Anson-Cartwright, L., Auerbach, A.B. & Joyner, A.L. Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2.
Hanks, M., Wurst, W., Anson-Cartwright, L., Auerbach, A. B. & Joyner, A. L. Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2.
