Sentence examples for mutant release of from inspiring English sources

In the E204Q mutant, release of the proton to the extracellular side occurs late in the photocycle with decay of the M2 intermediate, which results in accumulation of this intermediate.

Surprisingly, E1A-MEF expressing the murine Cox-2Y371F mutant released comparable amounts of PGE2 as E1A-Cox2-MEF.

Similarly, a pqsA mutant releases low levels of extracellular DNA and forms flat, thin unstructured biofilms with increased sensitivity to detergents.

To examine whether the dimerization of tetherin is important for its antiviral activity, we first generated tetherin mutants with cysteine to alanine substitutions (Figure 1A) and analyzed the effects of exogenous expression of these mutants on release of Lassa Z-induced VLP.

The development of molecular biology tools for Z. bailii, such as the isolation of stable auxotrophic mutants and release of a set of vectors allowing ectopic gene expression, is also relatively recent.

DOI: http://dx.doi.org/10.7554/eLife.01579.011 We determined whether the motB1 and flrA mutants released less of this signal molecule than wild-type V. fischeri using the Limulus amoebocyte lysate (LAL) assay to quantify the amount of reactogenic LPS in cell-free supernatants prepared from exponentially growing cultures.

Also, upon addition of ATP to prebound F-P5-mtHsp70 complex, the P486S mutant exhibited the release of bound F-P5 to comparable level as the wild-type (P < 0.05; Fig.  7D).

At 7 h post infection of HeLa cells, the point mutants released intermediate levels (∼60%) of LDH, not as high as the fully toxic strains but not as low as the effectorless type III-competent strain, ΔUT+pUCP (gray bars, Fig. 6C).

We created a degP mutant and observed a release of proteins at 37° to the culture supernatant similar to that of virK.

Only the H276A mutant failed to catalyze release of polyketide products and yielded 7 as the major product upon base hydrolysis.

The DGJ treatment resulted in release of mutant alpha-Gal A from the chaperone BiP and in its conversion in the mature lysosomal form.