Sentence examples for mutant reacted with from inspiring English sources

When it was observed that β-mercaptoethanol (β-ME), which is required to maintain the catalytic activity of soluble IN (a F185KC280S mutant), reacted with CAPE isothiocyanate functionality to form the corresponding hydroxyethylthiocarbamate, a variety of mutant IN were examined which did not require the presence of β-ME for catalytic activity.

Cysteines at positions 116, 117, 120, 122, 123, 127, and 133 reacted extremely slowly with 4-PDS, and mutants F115C/, T118C/, L119C/, V129C/, V130C/, and R135C/Basal mutant reacted with 4-PDS faster than those of F116C/Basal mutant.

Under denaturing conditions (4 M urea), both the wild-type and P1040C/F1053C mutant exhibited little reactivity while the F1053C mutant reacted with DTNB.

The buffy null mutant reacted to starvation with the expected responses such as inhibition of target of rapamycin (Tor) signaling, autophagy initiation and mobilization of stored lipids.

LigB-12, the F1053C mutant, and the P1040C/F1053C mutant were reacted with 5,5′-dithiobis 2-nitrobenzoic acid) (DTNB) to investigate the presence of free cysteines.

In order to test the reactivity of the single-cysteine DARPin mutants, reduced mutants were reacted with a weakly thiol-reactive reagent under mild conditions, namely 1 mM 2-bromo N- 2-methoxyethyl)acetamide (BrAcEGMe, 5 eq). iN- 2-methoxyethyl1 h at 4 °C.

The ascorbate deficient vtc2-1 mutant reacted to high light conditions with a surprising strong increase of ascorbate in most cell compartments during long and short time exposure, despite alterations in ascorbate synthesis [ 61].

Parkin mutants were reacted with UbVS by mixing 0.14 mg/ml protein with 0.1 mg/ml probe, in Parkin reaction buffer (50 mM Tris (pH 7.4), 200 mM NaCl, 10 mM DTT).

When 2 ng of hgDNA was reacted with mutant probe 2789+5G→A-MUT25C (probe 12) to form a 1 bp T G mismatch triplex, the triplex-associated fluorescent emission was 100% lower than that emitted by the reaction mixture containing the perfectly matched triplex after 5 min incubation and remained constant thereafter (Figure 3C and Table 3).

As expected, all these R mutants but wa** [11] reacted with Baro-2.

Our results showed that NCM and its mutants could react with antibodies specific for 6HB and MPER of gp41, suggesting that these antigens are in the form of a trimer of heterodimer (i.e., 6HB) with three exposed MPER tails.