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The sctt mutant provided a unique opportunity to perform a structure-function analysis at single amino acid resolution of this evolutionarily conserved motif within an intact developing organism.
Although the arm mutant phenotypes were not considerably altered by loss of GSK3, the mild phenotype of the armF1a, GSK3 double mutant provided a sensitized background in which we could further assess the role of aPKC in morphogenesis.
The use of this rhodopsin mutant provided a number of advantages.
The pin2-1 mutant provided a contrasting control as, consistent with expectations, its sensitivity to IAA and NAA was distinct from wild-type.
Interestingly, the E17K/K179M-AKT1 double mutant provided a similar degree of protection as the E17K-AKT1 single mutant, suggesting that this cancer-associated PH-domain mutant can mediate survival signals independent of its catalytic activity in certain cellular contexts.
Use of the catalytically inactive AtPPC3 mutant provided a direct means of studying the kinetic effects of the Ser phosphomimetic BTPC mutation within the chimaeric Class-2 PEPC complex.
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Our work confirmed that this mutant provided an excellent negative background, enabling the assay of peptide-dependent QS activation without interference by endogenous CSP.
The genetically and phenotypically defined mutant provides a vehicle for investigating the important genes responsible for non-race-specific resistance to two distinct diseases.
The mutant provides a useful tool for investigating the important genes responsible for non-race specific resistance to two distinct diseases.
This GFP151TAG mutant provides a rapid qualitative assay of amber-suppression efficiency in E. coli; it has been demonstrated previously that at position Tyr151 introduction of unnatural amino acids does not interfere with the fluorophore or affect the correct folding of the protein [17].
Thus, the ldox mutant provides a system that has leucoanthocyanidin but not cyanidin.
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