Sentence examples for mutant proteins containing from inspiring English sources
We choose codons for amino acids Arg, Leu, Ser, Ala and Gly which are rarely used and compared translation of the modified D1 proteins with the respective mutant proteins containing insertions with frequently used codons.
The aggregation propensities for a series of single-chain variable fragment (scFv) mutant proteins containing supercharged sequences, salt bridges and lysine/arginine-enriched motifs were characterised as a function of pH and ionic strength to isolate the electrostatic contributions.
The first monitored the calpain cleavage of numerous mutant proteins containing internal deletions within the CCR (Fig. 2).
As expected, nuclear localisation was maintained in the wt protein and was rescued for the KNRQ and 8/K mutant proteins containing a TAg NLS (Fig. 4B, C).
Aggregation and cytotoxicity of mutant proteins containing an expanded number of polyglutamine (polyQ) repeats is a hallmark of several diseases, including Huntington's disease (HD).
However, suppression of normal termination codons could affect studies of mutant proteins containing for example fluorescent amino acids in living cells (in this case leading to a high background).
Each series of mutant proteins contained also bacteria transformed with the pKK388.1 vector lacking the CBS insert as a negative control, and the wild-type CBS that was used to normalize data.
R506 has also been changed to E and A. All mutant proteins contain a C-terminal hexahistidine fusion, which has allowed a simple one-step purification procedure using nickel chelate affinity chromatography to be developed.
On the other hand, mutant protein containing only RRM1 exhibited strong binding activity to the JCV promoter (Fig. 6C).
However, the suppression efficiency of this pair in mycobacteria is low (the yields of mutant protein containing an unnatural amino acid are less than 9% that of wild type protein) indicating that the suppressor tRNA does not efficiently compete with release factor in this system.
It is conceivable that the mutant protein containing the additional 59 amino acids is unstable because of some combination of protein misfolding or aggregation, leading to its degradation.
