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To date, experiments exploring the consequence of PKD activity for TCR function have used transgenesis techniques to target an activated PKD mutant protein (comprising the catalytic domain of PKD1) to either to the membrane or cytosol of pre-T-cells.
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Two mutants were found to have the transposon inserted in different locations within XC_0250, which encodes a protein comprising a putative cyclase (CYC) domain linked to an uncharacterised domain with tetratricopeptide repeats.
Since the first publication in 1998 of the structure of a recombinant soluble protein comprising the ligand-binding domain of GluA2 extensive studies have afforded numerous crystal structures of wildtype and mutant proteins including different ligands.
ST77 is a transgenic line expressing the 330 kDa human thyroglobulin protein (hTG); ST111 is a transgenic line expressing a 75kDaa protein comprising the human myelin basic protein fused in frame to the Reovirus Sigma1 protein (hMBP-Sigma); and 764 is a transgenic line expressing a 28 kDa mutant, nontoxic form of a staphylococcal subunit vaccine protein (mSEB).
Adjacency Lists of proteins comprising the protein-protein interaction networks.
Mice receiving RNAi treatment stopped producing the mutant protein.
Overexpression of this mutant protein is lethal.
A few months earlier, I flunked a genetic test and learned that I inherited a mutant protein from my father.
We were unable to detect a trapped carbamate at K262 in the K268A mutant protein or a trapped carbamate at K268 in the K262A mutant protein by MSMS.
Cells expressing the mutant protein showed no obvious growth defects at 32 °C and 37 °C (Fig. 4c).
DCM results from abnormal function of a mutant protein, which is associated with myocyte Ca2+ desensitization.
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