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The reduction in the small RNA complexity in nrpe libraries was minimal compared with prior reports of substantial reductions in an Arabidopsis rdr2 mutant (proportion of distinct = 0.04 or 0.05) (Table S1 ).
In some mutation accumulation lines, fitness became too low to accurately measure (final mutant proportion <10%) and thus these data have been excluded from all analyses except those pertaining to Figure 2 and the decay in average fitness over time.
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Finally, the pooled mutant proportions were each compared to the proportions of a control group in which the untagged and the HA-tagged wild type versions were pooled.
Skeletal muscle tissues and the established myoblasts showed quite high mutant proportions (~100 %), whereas blood tissues showed relatively low mutant proportions (~50 %).
Diehl et al. determined the number of adenomatous polyposis coli (APC) gene fragments in cfDNA of advanced colorectal cancer patients and found elevated mutant proportions in patients with advanced tumor stages 16.
The system of CRISPR/Cas9 cleavage in vitro showed a high accuracy similar to traditional T7 endonuclease I (T7E1) assay in estimating mutant DNA proportion in the condition of normal frequency.
Interestingly the Mutant Allele Proportion (MAP) was sometimes as low as 10%.
For each mutant, the proportion of receptor at the cell surface was not significantly different from wild-type, even though the upper band was partially or fully absent.
In the rad2C65Δ tpm1Δ double mutant, the proportion of enlarged cells was additively increased to approximately 28% of the population (Fig. 6B), and some enlarged cells were significantly larger than those of the rad2C65Δ and tpm1Δ single mutants (Fig. 6D).
Whereas in the wild-type strain, the frequency of cells exhibiting centromere coupling begins to diminish by three/four hours in meiosis (Fig. 1 C), in spo11Δ mutants, the proportion of cells with non-homologous coupled centromeres continues to rise and persists at all times evaluated (Fig. 3A, spo11Δ).
Mutant Allele Proportions (MAPs) ranged from 9% to 80%.
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