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Combining wild type and mutant profiles of these transcripts demonstrates the activation of a complex genetic program that delineates symbiotic nitrogen fixation.
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Subsequently, we searched the metabolites that contributed to this separation of the mutant profiles group from those of WT (discriminative metabolites, hereafter) (Additional File 1).
GI profiles have been used to predict gene function with high confidence, while the degree of similarity to other mutant profiles can reveal how groups of gene products cooperate in higher-level biological processes [ 34, 42, 60, 61].
This applies to the mutant genetic profiles of CSC that are variable between subclones within individual patients with leukaemia (Anderson et al. 2011; Notta et al. 2011).
To better illustrate the contribution to the functional evolution from each mutant, the product profiles of all the mutants were subject to a clustering analysis based on relative abundance of the four sesquiterpene products.
This study for the first time provides a detailed analysis of the current standard single read approach versus overlapping read pairs in the application of mutant spectra profiling of viral samples.
To determine if there were any alterations in the overall extractability of non-cellulosic glycan epitopes in the fpgs1- 1 mutant, glycome profiling of the cell wall material (alcohol insoluble residues; AIR) isolated from 35-day-old inflorescence stems of wild-type and fpgs1- 1 mutants was conducted (Fig. 4a).
In both WT and mutant strains, the profiles of extracellular proteins secreted into the culture medium are similar to those of purified cellulosomes [ 29].
CD spectra of AcrB mutants and SDS denaturation profiles of AcrB mutants.
Interestingly, the three Pmp22 mutants exhibited distinct profiles of gene expression, suggesting different disease mechanisms.
We validated the model by simulating mutant networks and comparing our results with reported gene expression profiles of mutant phenotypes.
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