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In general, the mutant produced more propionic acid, with up to 10% increase in yield (0.62 vs. 0.56 g/g) and 46% increase in productivity (0.41 vs. 0.28 g/L h), depending on the fermentation conditions.
To examine if the mice infected with the tpx mutant produced more inflammatory response than those infected with the WT strain, which inhibited the growth of the mutant in the mouse organs, the spleen gross anatomy was observed.
As expected for a fur mutation, this mutant produced more siderophores than the wild-type strain and this production remained unaffected in response to increased iron levels (from 1 to 50 µM), as determined by using the chrome azurol S assay (data not shown).
As expected the R132C mutant produced more 2-HG than R132H mutant.
The results were almost the same (see Additional file 2), as the PGN_0274 mutant produced more biofilm than the wild-type.
The product mixtures from the two cultures resembled those from xylan medium: the wild-type cells produced significantly more acetate than the mutant (29 versus 18 mM, P < 0.0001) and the mutant produced more ethanol (28 versus 7.1 mM, P <0.0005).
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hpnN mutant produces more 2-methylhopanoids, possibly indicating some role for hpnN in hopanoid homeostasis.
Under microaerobic conditions, pflA- and pflB-mutants produced more ethanol (163% and 207%, respectively) relative to the parent strain, using glucose as a carbon source.
C. neoformans ssn8 mutants produced more abundant hyphae during sexual development processes.
Also it was noticeable that all septin mutants produced more than one germination tube per germinated teliospore at both temperatures (Fig. 10C and not shown).
The jaw-5D +/ spl-D double mutants produced more highly serrated and more deeply lobed leaves than jaw-5D or +/ spl-D (Supplementary information, Figure S5E-S5G), suggesthat that spl-D synergistically interacted with jaw-5D.
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