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In a continuous-culture flow-cell system, using gluconate minimal medium, the mucoid strain with acetylated alginate formed microcolonies and grew into heterogenous biofilms, whereas the acetylation-defective mutant produced a thinner and more homogeneous biofilm.
Afifi et al. (2014) used gamma radiation and ethyl methanesulfonate to obtain a mutated strain EMS-1, and found that the mutant produced a higher alkaline proteinase activity than the wild strain.
The DV2G460P mutant produced a delayed viremia (day 3 versus day 2; Figure 1).
The AYE-Δblp2 mutant produced a biofilm only twice thinner than AYE.
Alteration were first observed at tetrad stage, wild-type meioses produced four uniform spores, while the mutant produced a mixture of dyads, triads, and tetrads.
Although the differences in the melting temperature were small, the mutant produced a unique melting curve in the difference plot when compared to the wild type.
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Indeed, when nosN was deleted from the chromosome of the nosiheptide producer strain Streptomyces actuosus, the mutant produced an analogue of nosiheptide, which lacked the modification at C4 of the indole ring.
In 293 cells infected with Ad5 dl342, which contain an E1B-58K protencodedoded by the integrated adenovirus genome, the mutant produced an E4-25K protein which associated with the E1B-58K protein derived from the integrated genome.
Analysis of the Western blot using the O-antigen monoclonal antibodies indicated that the polymerase mutant produced an LPS consisting of a core region plus one O-antigen subunit.
Additionally, D53 acts as a repressor of strigolactone signaling, and a d53 mutant produced an exaggerated number of tillers to control rice plant architecture [ 9, 10].
In an S. aureus monoculture, the combination of HQNO and TO was bacteriostatic, indicating that the pqsL mutant produced an additional factor required for the bactericidal effect.
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