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Further investigation showed that the P474N mutant possessed a higher (13.3%, p < 0.05) specific activity.
The c68 (docs1 1) mutant possessed a nonsense mutation in the C-terminal part of the DOCS1 kinase domain.
It was found that the K17I mutant possessed a hydrogen bond interaction path Ile17-Asp15-Ile46-His13-Leu115-Gln117-Asp116-His114 while the L35P mutand possessed a Pro35-Leu115 -Gln117-Asp116-His114 Leu115)-Gln117-Aspath-His114 path, which is responsible for the observed conformational change of His114.
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If so, we reasoned that overexpression of a TRAF6 mutant possessing a defective RING finger (C70A), and therefore incapable of autoubiquitination, should not inhibit NFκB activation induced by co-overexpression of TAK1 and its activator, TAB1.
Their study also demonstrated the growth of an ARB resistant CHIKV mutant possessing a single amino acid substitution (G407R) localized in the E2 envelope protein.
In order to verify none of the spontaneous resistance mutants possessed a general growth defect, the growth rate was determined at 30°C in a gyrator shaker in LB [24], [58].
As a comparison, among the previously studied S/T protein kinase knockouts in N. crassa (Park et al. 2011b), 57% of the mutants possessed a defect in at least one of the growth/developmental stages, whereas 45% had overlapping defects in all three.
The H5N1 VN1203-PB2627E mutant possesses an amino acid substitution (Lys-to-Glu) at position 627 in the PB2 polymerase subunit.
dPANK/Fbl Drosophila mutants possess a neurodegenerative phenotype and a greatly reduced life span.
However, only mpk4 mutants possess a similar phenotype to mekk1 and have similar changes in gene expression.
After six rounds of combined mutagenesis and DNA shuffling, seven mutants possessing a total of six point mutations were identified.
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