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Genetic analysis was conducted by backcrossing oxt6 to the wild type and scoring the resulting F1 progeny for either tolerance to AT plus BSO, or for the distinctive dwarf phenotype seen in the mutant plants when grown in soil (see below).
Reduced levels of expression of AOX1A and RDR1 genes were observed in the gai-1 mutant plants when compared to the non-mutant Ler plants.
No OsPsbS2 product was found in OsPsbS1 knockout mutant plants when two different PsbS-antibodies were used, suggesting that such a product could not be detected by these antibodies.
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Compatible with this hypothesis, the complementation of the third whorl floral organs of the pi-1 mutant plants were observed when CsPI was expressed under the control of the AP3 promoter 5D3.
Consistent with the morphological phenotype, Chl a accumulation in the chlB mutant was drastically lower in short-day conditions than in the WT; Chl a accumulation was reduced to 37.8% (chlB_5 mutant line) and 44.6% (chlB_6 mutant line) in chlB mutant lines compared with WT plants, when they were grown under a 8-h L/16-h D cycle (fig. 3 B and C).
However, the opr3 allele becomes unstable when mutant plants are infected with a fungus (Chehab et al. 2011).
The etr1;ers1-3 mutant plants died without bolting when grown at 120 μE light, producing greater than wild-type numbers of leaves under this growth condition.
In Arabidopsis, there are three SEU like genes [ 23], and we found that loss of function in SLK1 and SLK2 confers salt and osmotic tolerance similar to LUH when mutant plants were subjected to the stress conditions.
Note that a weakened ABA-induced stomatal closing response in cpk mutant plants, as found here when applying 10 μM ABA, is likely the result of parallel ABA activation of R-type anion channels (Meyer et al., 2010; Sasaki et al., 2010; Imes et al., 2013) and a possible less-stringent CPK regulation of the SnRK2 signaling branch.
Mutant plants were reduced in size when grown in nutrient-poor mix of vermiculite: Redi-earth: sand (12 3 1), but grew to the same size as WT plants when raised on nutrient-rich Metro-Mix (MM).
Both phenotypes emerged when double mutant plants were grown at a photosynthetic photon flux density (PDF) well above 50-70 μmol·m-2·s-1 μmol·m-2·s-1 μmol·m-2·s-1
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Justyna Jupowicz-Kozak
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