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For one of the mutant plants derived from line 10, segregation of the mutation was followed in the next generation (T3).
M3 nrd2 1nrd2–1 and nrd2 2 (all K/K; H/H, respectively) mutant plants derived from accession Col-0 were crossed with wild-type mapping partner Landsberg erecta (L er) by manual pollination of emasculated L er flowers.
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To isolate the HD t) gene, a map-based cloning approach was employed using 479 F2 mutant individual plants derived from the cross between the hd(t) mutant (japonica) × Zhenshan 97 (indica).
First, we examined three different lines of Arabidopsis plants derived from the wrky17-1 mutant for the presence of the WRKY17 transcript using RT-PCR.
To determine whether the soil-surface rooting and the lack of root gravitropic response observed in the mutant are controlled by the same gene, we performed segregation analyses by using 85 F2 plants derived from a cross between the mutant and WT Nipponbare (Table 1).
We used the cup method to determine the soil-surface rooting ratio of F2 plants derived from a cross between the mutant and Kasalath, which has a soil-surface rooting ratio similar to that of Nipponbare (data not shown).
Numbers in (E-G) indicate averages ± S.D. To map the Srs5 locus on rice chromosomes, we performed linkage analysis using F2 plants derived from a cross between the Srs5 mutant (Oryza sativa. ssp. japonica) and Kasalath (Oryza sativa. ssp. indica).
The bfl1 mutant described in this paper was recognised initially among F3 generation plants derived from a cross between the iAc (pSK300, TT3-24-1-1) and Ds gene trap (DsG, pSK200, TT2-10-1-1) transgenic lines described previously by Upadhyaya et al. (2002).
To this end, we isolated 96 individuals with the albino phenotype from the segregating 412 F2 plants derived from the cross of Pei'ai 64S and tcm5 mutant.
As for the dag1gai-t6 double mutant, we analysed by PCR-based genotyping more than one hundred F2 plants derived from both the dag1 × gai-t6 and the reciprocal cross, but we were unable to isolate the dag1gai-t6 double mutant.
We determined the biallelic mutations for both TRY and CPC based on their clustered trichome phenotypes, finding that the TRY and CPC mutations were transmitted to T2 plants with high efficiency; 46.2%, 100%, 82.6%, 100%, and 100% of nontransgenic T2 plants derived from five T1 lines, respectively, were biallelic mutants for both TRY and CPC (Table 1).
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