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This model is supported by evidence that elg1 deletion exacerbates mcd1 mutant phenotypes, indicating that Elg1p function is critical for the ultimate maintenance of sister chromatin pairing.
All injected animals developed the typical tra mutant phenotypes, indicating that with the RNA supplement, only the larval phenotype could be rescued (data not shown).
However, mutant strains containing ndd1 mutant alleles lacking the Pkc1 phosphorylation sites did not fully re-capitulate the pkc1 mutant phenotypes, indicating that additional defects are associated with loss of Pkc1 activity.
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The genetic isolation of the FuI population and the predominance of early non-lethal mutant phenotypes indicated that the genetic variants responsible for the phenotypic variation are maintained due to population isolation.
Our biochemical data and the analysis of mutant phenotypes indicate that ALS8 is due to DVAP loss-of-function, with Sac1 being downregulated and PtdIns4P levels subsequently increasing.
Functional studies using mouse Sox B1 genes to rescue fly mutant phenotypes indicate that the mammalian proteins are functionally related to both Dichaete and SoxN[ 12, 13].
Although a partial FIL transcript is produced by the fil-9 allele, the mutant phenotypes indicate that the T-DNA insertion most likely results in FIL loss-of-function and does not cause a dominant negative effect.
The failure of IBR5C129S to fully restore ibr5-1 mutant phenotypes indicates that IBR5 phosphatase activity is required for full auxin and ABA responsiveness, and it will be interesting to learn if any of the MAP kinases implicated in hormone responsiveness are IBR5 substrates.
The ETR1(C6S, C6S), ETR1(C4S) and ETR1(C6S) mutants were transformed into an etr1-6 etripleein4-4 Arabidopsis triple mutant background, their ability to rescue the mutant phenotype indicating that they are functional receptors.
All the F1 progeny, after crossing FN026 and FN028 with the wild type, displayed the mutant phenotype, indicating that the phenotype occurs in a dominant manner.
When crossed with fil-8 [ 27, 32] homozygous plants, all F1 progeny showed the fil mutant phenotype, indicating that the newly isolated mutant was allelic to fil.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com