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The genetic mapping data and mutant phenotypes described below suggested that mutants 5L/11S, 7Y and 13U represent novel loci.
The mutant phenotypes described above are associated with the absence of PHF cell contributions and failure of PHF cell programming towards a cardiogenic fate.
There are, for example, important differences between the sec8 mutant phenotypes described here and the phenotypes previously described for Drosophila Sec5 mutants with regard to NMJ formation [ 7, 22].
In addition to the expected hoxb1b mutant phenotypes described above, we unexpectedly found that hoxb1b -/- embryos exhibit abnormal hindbrain architecture with a discontinuous lumen at the level of presumptive r3 and r4 (therefore termed r3/4) (Fig. 1A,B).
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The mutant phenotype described here is characterised by defects in both the AS and the DME cells.
In the case of mouse, out of a total of 81 orthologs for which knock out and mutant phenotypes are described (see Materials and Methods), 75 (92.6%) exhibit defects ranging from mild to gross abnormalities, including lethality (Additional file 15).
Besides the aluminum sensitivity phenotype, both docs1 mutants shared also several root phenotypes described previously: less root hairs and mixed identities of the outer cell layers.
functions of the CSLD family of genes are not fully characterised, there have been a large number of phenotypes described for CSLD mutant lines.
The characterization of enz mutant embryos and larvae presented here may provide clues to the mechanisms behind phenotypes described for organellar biosynthesis mutants and morphants described previously.
The yeast phenotypes described above could potentially reflect a loss of cohesion-dependent activities in the cancer-mutant-containing strains.
These mutants display neurogenic phenotypes in embryos and duplications of the smaller sensory bristles (the microchaetae) on the thorax in addition to the types of phenotypes described earlier.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com