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ASPCR also can provide an accurate quantitative result of mutant percentage in a mixed population, as described (11 ).
For each mutation LOD, the associated mutation percentage was estimated by fitting a non-linear model between ∆Ct values and mutant percentage.
The AR positive rate was 73.3% for the 1% cut‐off level, 69.2% for the 5% cut‐off level, 69.2% for the 10% cut‐off level and 71.4% for the 20% cut‐off level of PIK3CA mutant percentage.
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Despite the flowering phenotype of brm mutants, a percentage of the mutant plants never flowered under non-inductive conditions what indicated that other players were also involved in brm flowering phenotype.
Quantitative PCR analysis revealed a low mutant allele percentage of 15%and10%0% in cases #2 and #3, respectively.
In the InvBE rep mutant the percentage of trapped Inv-fragments increased from 15 to 79% after 1 h in LB and, as in the InvA rep mutant, this increase was transient.
As in p5cs1 single mutant the percentage of large germinated pollen grains is not significantly different from that of wild type pollen grains, the 12% reduction of germination efficiency must be accounted for by the small and degenerated pollen fraction.
We have also added a new histogram showing that in the mutant, the percentage of cells with 53BP1+ foci are much higher in BrdU+ VZ cells compared to BrdU- cells in VZ or CP. 4) Recently, a few non-coding RNAs or splicing variants have been identified in REST locus from many human cancer cells.
Consequently this technical limit was likely to prevent mutation detection in these particular cases by Sanger sequencing.Similarly, in case #4 identified as IDH1 R132C by PCR but WT by Sanger sequencing, a low mutant allele percentage (14%) was the probable source of conflicting results between these 2 tests as the mutation was also detected by pyrosequencing.
To generate the mutants a percentage of 100%% was also used in FMTS for all operators.
Mutant survival percentages were normalized to their survival at 5 days without rapamycin (but vehicle alone).
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