This analysis reveals that the leo;luc function is required during development and the mutant patterns are not caused by pattern instability.
Mutant patterns were generated by setting the levels of production of the relevant TF to zero or, for Gli /–, by setting the binding affinity of Gli to zero.
The priors were then updated with the posterior distributions obtained from this fit and then the WT and mutant patterns were used together as targets; this was used to speed up the search process (the total time to obtain the approximate posterior distributions was around 3 weeks running on a single GPU).
In the stronger armXM19 mutant, patterning was abolished, all cells had a similar shape, secreted a denticle, and the planar distribution of all markers was uniform around cell margins (Fig. 2 B-B"').
A mutant expression pattern is then associated with a lost CNS or a cluster of CNSs, and these previously mysterious sequences acquire a testable hypothesis as to ancestral function.
It may be important that the mutant pattern strength is revealed not as an elimination of errors, but rather primarily as a resistance to disruption by errors.
Although ventral cell types were generated in the Shh Gli3 double mutants, the patterning is somewhat disrupted, in particular the strict DV organization characteristic of the normal neural tube is less evident (Litingtung and Chiang, 2000).
She has seen cases in which scientists have failed to realize that their genetic mutants — for instance, Arabidopsis mutants with altered branching patterns — are recapitulating naturally occurring plant forms found in other lineages.
In the double mutant, BSEP circadian pattern is markedly altered, but its expression level on average is not different from that of the wild-type.
In the weaker armF1a mutant, some patterning was maintained, and cell shape changes were only mildly disrupted (Fig. 2 D-D"').
