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Mutant p53 was downregulated.
Mutant p53 was first cloned from p65 null cells by RT-PCR.
In early studies, expression of mutant p53 was demonstrated in breast cancer cell lines [ 6].
Survivin levels were higher when defective mutant p53 was expressed at 39°C, and radiation did not reduce these levels.
R213* encoding mutant p53 was isolated from a patient with RA and has previously been characterized as dominant-negative [ 14].
The expression plasmid of mutant p53 was a gift of Dr. Jennifer Pietenpol, Vanderbilt University, Nashville, TN).
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Interestingly, mutant p53 is easily detected via immunohistochemical analysis [ 14].
One mutant, D168W, was created.
The mutant H222D was inactive.
Mutant 1X-1 (Q122R) was harvested from directed evolution.
The mutant AroG15 was demonstrated to a thermostable mutant.
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