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Many of the klp10A knockdown and mutant oocytes, however, showed cortical microtubules that were brightly decorated with EB1-GFP.
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However, jip3/syd mutant oocytes show no defects in the posterior localization of Dynein (Palacios and St Johnston, 2002).
However, in bubR1 mutant oocytes, SMC2 localizes within the nuclear space rather than being bound to the karyosome.
Paradoxically, however, endos homozygous mutant oocytes show normal levels of Cdk1 kinase activity in in vitro assays (Von Stetina et al. 2008).
However, most of these mutant oocytes show an extension of this anterior 'bright' MT network towards the posterior around the nucleus (supplementary material Fig. S6F,G), as well as the misorganization of MTs in a pattern that resembles the aberrant vesicles often detected at the anterior of Khc mutant oocytes (supplementary material Fig. S6F,I, arrows; vesicles in Fig. 6).
The ovaries in Hr39 and lz mutant females contain a full complement of mature oocytes, however, both lay significantly fewer eggs than controls, indicating that secretory products are required for one or more steps downstream from oocyte completion, such as ovulation, mating, sperm storage, fertilization, or egg laying.
However, the spindles that form in lin-41 mutant oocytes have obvious astral microtubules and therefore resemble mitotic, rather than meiotic, spindles, suggesting that centriole elimination has not occurred.
However, oskar mRNA (detected by Staufen antibodies) is never localized to the posterior pole in Khc mutant oocytes (Khc germline clones, GLCs) that express KHC1-849GFP (n=80, Fig. 1D), indicating that amino acids 850-975 arequiredred for oskar transport.
The genesis of spindle defects in the live mutant oocytes provides new insights into the mechanism of Ncd function in the spindle during the meiotic divisions.
We found that HSPA5 protein was more abundant in mutant oocytes and embryos.
Why are okra mutant oocytes used as a control?
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