Our observation that the not5Δ rrp6Δ double mutant on one hand, and not5Δ caf40Δ double mutant on the other hand (unpublished data) have severe growth defects is compatible with the idea that Not5 and Caf40 might participate at different steps in the functional connection of TRAMP and the nuclear exosome.
Because we were so surprised at not being able to replicate the functional expression of this mutant, we in fact constructed this mutant on three independent occasions, but in each case, the lack of fuctional expression remained the same.
We found that overexpression of LARK partially or fully suppressed night-time eclosion events for the E74BG01805 mutant population on two consecutive days of LD (Figure 4).
The results provide also an explanation for the dramatic difference between C. elegans and D. melanogaster unc-76 mutants on one hand, which cause serious defects in the nervous system, and the mouse FEZ1 -/- knockout mice on the other, which show no morphological and no strong behavioural phenotype.
To determine the role of laeA in A. niger in relation to secondary metabolite production, the production of secondary metabolites in wild-type and laeA mutants on three different media and culture conditions was analyzed.
Hartlieb et al. have identified mutants located on two opposite faces of the protein, both of which affected the ability of VP30 to co-immunoprecipitate the NP protein.
While pectin is a relatively minor component of the grass and woody dicot substrates, the reduced growth of the mutant on all three biomass types provides direct evidence that pectin plays an important role in biomass recalcitrance.
Conversely, a phospho-mimetic mutant on all three casein kinase I sites served as a better substrate for cdk5 when compared with wild-type parkin in vitro (540 ± 240%; mean±S.E.M., n = 3) (Fig. 3B).
The presence of small, cuboidal Asxxen mutant cells on one surface of the wing blade likely prevents proper apposition of larger, star-shaped wing blade cells on the opposite wing surface.
We found that functional inhibition of endogenous RARα in breast cancer cells by using either RARα specific antagonists or a dominant negative RARα mutant hampers on one hand the RA-induced upregulation of neutral sphingomyelinase (nSMase -mediated CER synSMase -mediatedthe other hand the RA-induCER downregulation of synthesisne kinand 1, SK1, pivonal for S1P synthesis.
