Even the isogenic comparison between GA20ox mutants, on both the Landsberg erecta and the Col background, show no trade-offs during drought.
A requirement of malignant cells to undergo metastasis is to acquire the ability to penetrate the surrounding extracellular matrix in order to migrate to distant tissues [ 22]; thus, we tested the effect of Rac mutants on both migration and invasion in vitro.
By differentiating the effects of PCC 7806 WT and its microcystin-deficient mutant on Daphnia, both of which are known to contain strong trypsin inhibitors [ 19], the negative effects of microcystin-producing cyanobacteria on Daphnia could only be assigned to this compound to a limited degree [ 44, 45].
Alternatively, since COMMD1 is involved in multiple physiological processes, mutant COMMD1 on both the alleles might cause pre-natal fatality.
The present study demonstrates the suppressive effects of cJun dominant-negative mutant, TAM67, on both anchorage-dependent and -independent growth of a NSCLC cell line as well as in vivo tumour growth.
Staining with a phosphotyrosine-specific PDGFRα antibody recognizing the activation loop tyrosine highlights the constitutive activation of the mutant proteins (on both receptor bands, Figure 1C, lanes 4 6) and confirms the stimulation-dependent activation of the wild-type receptor (on the upper receptor band only, highlighted by arrows in Figure 1C, lanes 2 and 3).
In a season of crime fighter against crime fighter, super power vs. super power films, this mutant-on-mutant spectacular has the most depth, innovation, majesty and visual wonder.
The cell density in mutant on the outer surface of both palea and lemma were significantly higher than the WT, with observed recovery in the C4 line (Fig. 2a c).
Germination ability was assayed by plating both wild type and mutant on nutrient rich agar, the mutant produced 5 ± 34% of wild type germinating spores.
