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Overexpression of ZRT2, improves the growth of the zap1_/ zap1_ mutant on a low-zinc medium [ 32].
In order to assess the effect of a tumorigenic MLK3 mutant on a genome-wide level, we performed microarray-based expression profiling experiments.
Cells were mounted in ProLong Gold (Invitrogen) and imaged under the same settings for each mutant, on a Zeiss LSM 700 confocal microscope using an αPlan-Apochromat ×100 objective.
In addition, heterozygote Gja1 Jrt/+ (Gja1 m1Jrt; also known as Cx43G60S/+) mice carrying the G60S mutant on a background of C3H/HeJ and C57BL/6J mice were used for primary culture experiments.
Further, to describe how the Genotype effect (Apc Wild-Type vs. Mutant) on a plasma metabolite concentration varies by the Diet (High Fat vs. Low Fat), or vice-versa, we focused on the Genotype by Diet interaction effect.
(E ) TES reactions were carried out with SsoCas1, or the E142A active site mutant, on a fork substrate containing a nicked SacI restriction site spanning the branch point (SacI substrate).
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The organic solvent tolerances of the mutants (CH1 to CH8) were investigated by measuring the colony-forming efficiency of each mutant on an agar plate overlaid with pure cyclohexane and a mixture of cyclohexane and p-xylene (7 3 vol/vol mixture) (Figure 1).
The pale tremor mutant, Fig4 −/−, is derived from a spontaneous mutant that arose on a mixed inbred background (3).
We applied the operators to 12 small AspectJ applications and ran the non-equivalent mutants on a functional-based test set.
The review critically summarizes procedures for the isolation and purification of native and engineered eubacterial elongation factors EF-Tu and their mutants on a large as well as small scale.
If space marines cutting down mutants on a distant planet is just too real for you, the throwback-styled indie scene's got options aplenty for your bloodthirsty appetite.
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