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To date, the ABA biosynthesis and catabolism pathway has been completed by using different mutant of these pathways, genetic approaches and other feeding experiments.

Loss-of-function mutant of these mutants also display an ABA-deficient phenotype (Kang et al., 2010; Kuromori et al., 2010, 2011).

A combined mutant of these three subunits can, in fact, still be loaded onto double-stranded DNA, albeit with low efficiency.

However, neither the Ala nor Asp mutant of these two residues is sufficient to abrogate binding of TIMELESS to PARP1, suggesting that their phosphorylation may not play a role in regulating their interaction.

For example, although neither the ERG11A gene nor the ERG11B gene in Aspergillus fumigatus is individually essential, the deletion mutant of these two genes is not viable in immune-compromised mice, suggesting the pair of genes as a combined drug target [ 67].

We defined a list of candidate genes that could play a role in fiber development (Table  4), their specific transcript expression in fiber containing tissues and mutant of these genes should be analyzed in detailed in future studies, and these genes area target for manipulation through reverse genetics.

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Similar(53)

To test this hypothesis, we generated in silico mutants of these basic residues in Bad peptide and Mcl-1/A1 proteins.

Phenotypic analysis of loss-of-function mutants of these genes showed that they play distinct overlapping and antagonistic roles in development.

At least in 49 to 87.88 % of the cases they are able to point specific source code lines containing mutants of these mutation operators.

PAC from Escherichia coli strain ATCC 11105, Bacillus megaterium strain ATCC 14945 and mutants of these two strains is currently used in industry.

Representatives of all 10 motifs were found to display enhancer activity in vivo, whereas point mutants of these sequences exhibited sharply reduced activity.

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