The total fraction p of viable sequences in this model is equal to the expected fraction of neutral mutants of any sequence with fitness 1, and w(d) ≈ p for d > 0 in this model.
In temperature-sensitive mutants of any member of the septin family, polymerization does not occur, cytokinesis is blocked, and mitosis may proceed with the formation of multinucleated cells, a process that is similar to that forming some PGCCs.
We found that odor-evoked AWB activity was not affected in mutants of any of the three identified C. elegans muscarinic receptors (gar-1, gar-2 and gar-3) (data not shown), suggesting that acetylcholine might bind other receptors on AWB neurons.
Furthermore a mutant of CAgp130 without any cytoplasmic Tyr-residues was generated – CAgp130-6F-YFP – to serve as a negative control.
We were unable to recover viable double mutant combinations of any of these three genes (tba-2 deletion alleles were not available to include in this analysis).
Most of the chromosomal regions devoid of mapped genes with a mutant phenotype are nevertheless populated by loci that were included six years ago in a comprehensive dataset of all known Arabidopsis genes with a mutant phenotype of any kind [14].
We studied respectively Escherichia coli K12 mutant devoid of any cell surface appendage [43], and performed single-molecule force spectroscopy (SMFS) experiments on Pseudomonas fluorescens because of its exopolysaccharide production.
Flies homozygous mutant for any of the four Sans alleles were adult viable, male and female fertile, and had no gross morphological defects (data not shown), indicating that Sans is not essential for fly development and fertility.
In standard single-pair courtship assays, males mutant for any of the three isoforms still courted virgin females, but with reduced copulation success compared to wild-type control males.
