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In the context of a PDGFRα mutant occurring in glioblastoma, aberrant intracellular localisation was detected [ 25].
In each cycle, the probability of exactly one mutant occurring in cycle t is (5) which is about p t × μ if we assume that μ is small.
If a haplotype representing the first mutant occurring in one of the first k−1 marker populations had t a < 1, this mutation was allowed to have an initial frequency greater than q0, the initial frequency of the corresponding wild-type haplotype being decreased accordingly.
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This mimics the situation where a random WS mutant occurs in a population and rises to high frequency without clonal interference from other WS mutants.
Although the mutant occurred in a strain from the Ahringer RNAi library [ 38], the mutation occurred during culture in our laboratory.
The results confirmed that the desilencing in the hrde- 1 mutant occurs in the germline tissue for these genes (Additional file 2).
A potential disadvantage of working with natural populations and the mutants that they contain is that each mutant occurs in a different genetic background, and that this background can influence observations.
The reduction of monomeric Lhcbs in the chy1chy2lut2lut5 mutant occurs in spite of the presence of the lut2 mutation, which favors LHCII monomerization (compare band 2 in wild type vs. lut2 and in chy1chy2lut5 vs. chy1chy2lut2lut5, Figure 5B).
In this study, we set out to compare the signalling capacities and transcriptional responses of different oncogenic PDGFRα mutants occurring in GIST patients with those of the wild-type PDGF-receptor.
It has to be noted that there is limited data definitively linking these genes with the hyper-invasive phenotype with only one of our previous transposon mutants occurring in this locus [ 16].
Since the mutation in tcd10 mutants occurred in the 5th PPR motif site (Fig. 3e), resulting in the changes of structure and loss-of-function of TCD10, thus the tcd10 mutants should loss all three functions aforementioned.
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