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'Meh0106' is a homozygous FHB-susceptible mutant obtained from M2 progenies of Wangshuibai with dry seeds treated with 0.35% (w/v) EMS.
n=4 wild type + 4 Cdkn1c mutant, obtained from two litters (2+2 for each litter).
Interesting to note is that the mutant obtained from this study also overexpressed eight oxioreductases that can use NADPH as an electron donor.
This set also included parental genotypes of published mapping populations, namely JI281, JI399 and JI15 [ 45], cv 'Terese', K586 which is a mutant obtained from Torsdag [ 46, 7] Champagne [ 7, 20], JI296 and DP [ 12].
The receptor mutant, obtained from Dr. Kazlauskas and collaborators, lacks the extracellular ligand-binding domain and is therefore unable to bind or be activated by proper PDGF ligand(s) or other signaling molecules [16].
Also, there is a possibility that there were DNA sequence specific differences between the A. thaliana FN1148 Col-0 mutant (obtained from J. Schroeder's lab, UC San Diego, USA) and the control A. thaliana Col-0 line that had been propagated in our own laboratory since the 1990's.
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Previously, we constructed a S. cerevisiae strain accumulating approximately 120 mg/g-Dry cell weight RNA using suppressor mutants obtained from rrn10 disruptant (Chuwattanakul et al. 2011).
In this work, we have analyzed the stability of protein mutants using two different datasets of 1396 and 2204 mutants obtained from ProTherm database, respectively for free energy change due to thermal (ΔΔG) and denaturant denaturations (ΔΔGH2O).
If our results were to be duplicated using aspartate mutants obtained from site directed [39] or random mutagenesis [33] this would strengthen the tentative conclusions we draw below.
Experiments were carried out in BY4741 (MATa his3Δ 1 leu2Δ 0 met15Δ 0 urand 0) and respective null mutants, obtained from Euroscarf.
Experiments were carried out in BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 urasΔ0) as the wild-type strain and respective null mutants, obtained from Euroscarf.
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