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As flies aged, we began to detect fragmentation of the axons and dendrites of MB projection neurons both in wild-type and p35 mutant neurons using MARCM mosaics (Fig. 5).
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In this study, we analyzed the distribution and function of CAPS proteins in DCV trafficking and fusion in mammalian neurons using wild type (WT), CAPS-2 null mutant and CAPS-1/2 null mutant mice.
The six GFP-Parkin mutants were serially introduced into PARKIN−/− primary neurons using a lentivirus and assayed for their subcellular localization after CCCP treatment.
Neurons were transfected at DIV 9 with full-length IL1RAPL1 or mutants (IL1RAPL1▵C and IL1RAPL1▵N) and GFP to visualize transfected neurons, using immunofluorescence.
(A D ) P0 quantification of Cux1 expressing neurons using 8 Bin quantification in conditional mutants and control shown in Figure 6.
Rest mutant and control ES cells were differentiated into NSCs, NPCs and then neurons using a monolayer culture in N2B27 medium [19].
(D and E ) Quantification of the average number of commissures per embryo in comm mutants expressing the indicated HA-tagged receptor transgenes in either all neurons using elav-GAL4 (D ) or in midline cells using slit-GAL4 (E ).
Here we generated novel mutant mice (PDGFR-β KO) in which PDGFR-β was conditionally deleted in CNS neurons using the Cre/loxP system.
When expressed either in neurons using elav-Gal4 or in gut using c601-Gal4 or NP1-Gal4 (data not shown) dGPHR could rescue the dGPHR mutant growth defects (Fig. 5B).
To manipulate the BNST neurons, the researchers used a technique called optogenetics that allowed them to activate specific neurons using light.
The two rationally generated mutants A56P and TP [ 22], the inherited A30P mutation, human WT αSynuclein, and EGFP were expressed in cultured primary cortical neurons using AAV2 vectors.
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