Sentence examples for mutant missing the from inspiring English sources
To determine the role of each residue of AChE586-599 in the process of amyloid formation, a library of alanine scanning mutants along with a structurally conserved substitution mutant (Tyr to Phe) and a truncation mutant (missing the last residue) were used.
Both halves of POSH pulled down full length c-Cbl, Cbl-b, and Cbl-b 2/3 (a mutant missing the N-terminal TKB domain).
The SUMO localisation likely corresponds to SUMO1 conjugated to substrate proteins because a non-conjugatable SUMO1 mutant missing the last two amino acids (SUMO1ΔGG) does not form an INB (Desterro et al. 2005; data not shown).
The remaining eight lysines were each individually mutated to alanine and tested for crosslinking with Fis-Q21C; a truncation mutant missing the two C-terminal lysines was also evaluated.
However, PML VI (NP_150247.2), which does not have the SIM, a mutant missing the SIM, or PML 3 KR, which has lost all three SUMOylation site, are still capable of forming nuclear bodies and form PML polymers [ 16].
(C ) Western blot showing the recovery of endogenous OCRL in immunoprecipitates from cells expressing GFP-SNX9WT but not from cells expressing GFP-SNX9 mutant missing the SH3 domain (GFP-SNX9ΔSH3).
The mutant misses the C-terminal ERM-binding tail, therefore it is not able to bind to ERM.
The structure of the LPS can be partially deduced by its size following electrophoresis because it is assembled sequentially and as a consequence, mutants missing the core or O-antigens will migrate faster.
As shown in Figure 4C, all mutants missing the C-terminus were quite stable in response to γ irradiation, while the wild-type and all N-terminal mutants were degraded in the extract prepared from the cells treated with γ irradiation.
In this way, single mutants missing the first (N70, 'NQ1'), second (N119, 'NQ2'), third (N243, 'NQ3') N-glycosylation site or double and triple mutants missing two ('NQ12'NQ13Q13'NQ23Q23') or all three ('NQ123') sites were generated.
Together, these findings may suggest that the ZF1 mutants missing the zinc-chelating residue are recognized as misfolded proteins and trapped by a molecular chaperone or degradation system (48, 49).
