Sentence examples for mutant minigenes based from inspiring English sources

Wild-type and mutant minigenes based on TauEx9-11 were subcloned into pCIneo.

Our preliminary results from co-transfection of mutant minigenes and PTMs indicate successful, but weak trans-splicing at the RNA level.

Wild-type and mutant minigenes were transfected into HeLa cells using lipofectamine (Invitrogen).

HEK293 cells were transfected with 500 ng of wild-type or mutant minigenes using Lipofectamine Life Technologies Ltd , Paisley, UK).

GH3 cells were transiently transfected for 24 48 hr with WT and mutant minigenes separately or together in equal amounts.

This showed that there was no difference between the wild-type and mutant minigenes with both producing mRNA with normal inclusion of coding exon 15 (Supp. Figure S4b).

Wild-type and mutant minigenes L1-GHR8-L2 were subcloned from the bacterial pGEM T-easy vector into the mammalian pcDNA 3.1 vector.

The pBsKFIX exon 5 wt and mutant minigenes were expressed in BHK cells with or without the modified U1 snRNAs complementary to the F9 donor site (U1FIXwt) or to the downstream intronic sequence (fix9).

Transfection of the five pBsKFIX mutant minigenes showed complete skipping of the exon (Fig.  7B, lanes 2, 5, 8, 11, 14), and cotransfection of either U1FIXwt or fix9 ExSpeU1 induced a complete rescue of the splicing pattern.

This is often tied in with mutant paranoia based on some item of religious doctrine, however small or seemingly inconsequential.

WT and mutant minigene constructs were prepared and fragments were cloned into the EcoRI site of pcDNA3.1+ (Invitrogen, Paisley, UK).