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Since we also found that gemin5's m7G-binding capability maps to the WD repeat domains, we tested whether the W286A mutant might affect cap-binding.
Thus, the α SNAP LOOP mutant might affect selectively binding to either Stim1 or Orai1, which is important to test.
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It will be interesting to test whether other BR-deficient mutants might affect expression of these grain size genes in the future.
Thus, these mutants might affect intramolecular interactions or the overall 3-dimensional structure of the PTP-PEST protein, thereby affecting autoregulatory functions [67].
To determine if these mutants might affect the stability of STBD1, we generated stable Hela cell lines of STBD1 proteins or GFP (green fluorescent protein) as a control.
DOI: http://dx.doi.org/10.7554/eLife.00220.012 We next asked if removing membrane anchoring of LRP4 (as in Lrp4ECD/ECD mice) or deleting App and Aplp2 (as in App/ Aplp2 double mutant mice) might affect LRP4 protein distribution in muscles.
Considering that Fgfr2 is expressed in the outer layer of immature osteoblasts of the palatine bones during development (Rice et al., 2003), a possible explanation that requires further testing is that altered growth kinetics bestowed by a mutant Fgfr2 might affect bone shape throughout development, causing the localized thickening of palatine bones and the wider separation in the midline suture.
We characterized the Δstn mutant and showed that Stn might affect membrane integrity via regulation of OmpA localization.
Chronological lifespan measurements of S. cerevisiae wild-type and mutant strains suggested that ROS might affect and potentially even determine the postmitotic lifespan of yeast (Longo et al., 1997; Fabrizio and Longo, 2007).
This might affect the functioning of mutant JNK2 in ways additional to the predicted effects on molecular access into the expanded ATP binding pocket of JNK2.
Given the effect of the DaPKC alleles on ept/M 3) tumor growth, we next examined whether DaPKC activity might affect Rbf1 levels in ept mutant cells.
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