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But it doesn't fall into the traps of contemporary design: mutant materials and sharp edges.
To facilitate quantitation (the anti-Osk antibodies have substantial background staining) we used an osk::HA transgene, which expressed osk under its normal transcriptional control and fully rescues an osk mutant (Materials and Methods) (Kim et al., 2015).
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We therefore proceeded to evaluate the essentiality of each RNR class during the infectious process, first constructing nrd mutants for each RNR class and then combining them to obtain double mutants (Materials and Methods).
MGE explants were collected from 4 different genotypes: control, Rac1 mutant, Rac3 mutant, and double mutant (see Materials and Methods section describing mice).
We thank Dr. B. M. Khadi and Dr N. Gopalakrishnan for providing MCU5 and its near isogenic fuzzless-lintless mutant materials during the initial studies and for their helpful discussions.
CHS and WQL provided the mutant materials.
She foresaw this change in her groundbreaking 1995 show at the Modern called "Mutant Materials," which credited scientists with inventing the new materials that architects and designers use in unorthodox ways.
The designer's American debut was in the 1995 "Mutant Materials" exhibition at the Modern, which presented radical new materials appropriated by designers from the aerospace and other high-tech industries.
As long ago as 1995, decades away in Internet time, MOMA curators would put on other institutions' Web sites such exhibitions as "Mutant Materials in Contemporary Design," a look at artists working in materials like carbon-fiber-reinforced plastic.
Therefore, we decided to further characterize the remaining 8 mutants and swi3-NBT7, wasch was individually isolated as a mating-type switching defective mutant (see Materials and Methods).
As shown in Figure 8B, deletion of RAD9, RAD24, MRC1, CHK1, DUN1 or PDS1 (partially deleted as a temperature sensitive mutant, see Materials and Methods) promoted the proliferation of type I cells on plates containing bleomycin.
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