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Residues that are invariant amongst Doc1/Apc10 sequences, including a temperature-sensitive mitotic arrest mutant, map to a β-sheet region of the molecule, whose counterpart in galactose oxidase, the coagulation factor C2 domains and XRCC1, mediate bio-molecular interactions.
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Gon et al [19] found that suppressor mutations in a thioredoxin-glutaredoxin mutant mapped to an operon encoding dnaA and dnaN.
Another reported mutant maps to XcOGT D471, which hydrogen bonds the UDP ribose.
Notably, the importance of these N-lobe/C-lobe interactions is confirmed by impaired ligation activity for every Ala scan mutant mapping to this interface.
Distribution of mapped reads among genomic features showed that a high proportion (49% for both the wild-type and the mutant) mapped to protein coding regions indicating a high level of coverage of actual transcribed sequences (Additional file 6).
This mutant mapped to the right arm of chromosome II, but further mapping was prevented by the probable inversion in this region that likely resulted from the integration of the phat-1:: YFP transgene (see Materials and Methods).
To confirm that the causal mutation in the twr-1 mutant maps to the At5g55580 gene encoding a mitochondrial transcription termination factor (mTERF) family protein, we obtained a second allele harboring a T-DNA insertion in the fourth intron.
The s1 and s2 mutants map to SesA.
The SNP analysis revealed that 22 mutants map to only five chromosomal regions; only mr37 showed no Mendelian inheritance of the trait.
We show that in a collection of 136 randomly selected Ctf mutants, >65% of mutants map to 13 genes, 12 of which are involved in sister chromatid cohesion and/or kinetochore function.
Although such hypotheses are currently beyond experimental proof, it is interesting to note that several, structurally unexplained superactivity mutants map to this central sequence (for example, mjA' D816-N; Q817-S/T/C/K and V819-K Figure 2A[ 20]), and that this region is also exceptionally tolerant to radical twisting of the α-helical axis induced by deletions of two-amino acid segments.
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