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Although the Y494D mutant lost the DnaK-dependent disaggregation activity, the intrinsic disaggregation activity of this mutant was recently demonstrated to be high by using covalently fused yellow fluorescent protein aggregates as substrate34.
Similarly, the I167A mutant lost the activity, and the I167V mutant showed a significant decrease in the activity, to 24.5% of the wild type.
The deletion mutant, and the subtler D184N point mutant lost the ability to inhibit chlamydia growth (Figure 5).
However, when basic residue R395 was replaced by a non-basic residue, R395N, the mutant lost the binding efficiency.
We previously showed that the GSS-associated A117V PrP mutant with a valine at codon 129 retained the anti-Bax function while all of the 11 CJD-associated PrP mutations carrying a methionine at codon 129 or the FFI-associated D178N PrP mutant lost the ability to prevent Bax-mediated condensed chromatin in human neurons [31].
In accordance with this, the DBD mutant lost the activator effect of FoxO1 overexpression on apoptosis in MGCs.
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The sor1 mutant loses the root-specific gravitropic response at an early growth stage.
The primary roots of the soil-surface rooting mutant lose the root-specific gravitropic response at an early growth stage.
Interestingly, a p27E171* mutant, losing the last 28 aminoacids, has been identified as driver mutation in LBC [ 2].
However, the age-1(hx546) mutant loses the ability to compete with the wild type if the mixed population is exposed to repeated cycles of starvation and feeding [ 14].
Together with the finding that only the sgo1-700 mutant loses the bias to sister kinetochore biorientation, this provides strong evidence that Sgo1 biases sister kinetochores for biorientation through condensin recruitment.
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