There is direct visual evidence that SPI-1 mutants localize in the lamina propria but not in epithelial cells [27].
However, in vitro the deletion of RTDL motif inactivated MANF in the sympathetic neurons where the mutant protein localized to Golgi, but not in the sensory neurons where the mutant localized to the ER, showing that intracellular MANF protects these peripheral neurons in vitro only when localized to the ER.
So the SB mutant remains localized in both cases, either by a local collapse of the dendrite or because it is left behind by the escaping cells.
In contrast to the nuclear localization of wild type, RBM10 mutant predominantly localized in the cytoplasm (Fig 6D), consistent with the loss of NLS.
Consistent with earlier results (Bjørkøy et al., 2005; Ichimura et al., 2008; Pankiv et al., 2007) the wild-type and LIR mutant proteins localized in multiple puncta, but only wild-type p62 extensively co-localized with LC3B.
In the non-reversing frzlof mutant, YFP MglA+ localized in a cluster at the leading pole and did not display dynamic localization.
In contrast, the C-cut mutant was localized in the cytoplasm and lost ability to induce neurites and spines (Fig. 8A and B).
In addition, wild-type BAP1 mostly localizes to the nucleus, whereas the F170I mutant preferentially localized in the cytoplasm.
In the mglAG21V mutant, RomR GFP localized in a bipolar, symmetric pattern.
PTRN-1 GFP PTRN-1 GFProm its expresseds promoter (P ptrn-1) rescued the synthetic lethality ofrome noca-1∆; ptrn-1∆ double mutant and localitsd in multiplendogenous including the larval/adult epromoter, neurons, intestine, and Pharynx.
